Analysis & Consulting - RENTACS

Analysis & Consulting

Sampling for Legionella Research and Quantification

RENTACS Services

Water Analysis & Technical Consulting

RENTACS is the ideal partner to support you in risk management by offering you the expertise for a complete hygiene plan and for training and consultancy on water safety.

  1. Water Analysis
  2. Risk Analysis
  3. Hygiene Protocol
Analysis & Consulting

What does this include?

Water Analysis

We carry out environmental water sampling using the PCR method for the detection or quantification of Legionella spp. and Legionella pneumophila in all water samples in a few hours rather than the long incubation period required by traditional methods.

Risk Analysis

Legionellosis risk assessment is a legal obligation by national and regional guidelines. We do a technical inspection of the water system and do a risk assessment to produce a risk priority index and define the activities immediately necessary to reduce any risk.

Hygiene Protocol

Starting from the risk assessment document, we create a specific hygiene protocol for each plant with staff training, maintenance and monitoring plans and perform control audits on hydraulic and aeraulic systems.

Water Analysis

Bacteria of the genus Legionella are opportunistic pathogens, commonly spread in aquatic environments: springs, rivers, lakes, thermal waters, etc.

From these environments they can spread in artificial works such as drinking water pipes and building water systems, fountains, swimming pools, tanks in which the bacterium can develop.

Traditional

We carry out environmental sampling of drinking water, boiler water, cooling tower water, filters and condensate of air conditioners, medical and industrial water, certified by the ACCREDIA laboratory, with quantitative counting of the UFC (Cologne Forming Units) and research of the type (Serum group).

Samples comply with the guidelines and legislation in force.

We carry out sampling for Legionella research and quantification with Real Time PCR technique, certified by the ACCREDIA laboratory, with quantitative counting of UFC (Colony Forming Units) in less than 24 hours.

PCR Method

Traditional methods for the detection of Legionella spp and Legionella pneumophila in water samples have the disadvantage of having a long incubation period. The research of the same with the PCR method allows to obtain results in a short time and with greater sensitivity.

These methods allow for the detection or quantification of Legionella spp. o Legionella pneumophila in all water samples in a few hours, in which the following steps take place:

1. Sample filtration
2. DNA extraction
3. PCR reaction for the detection of Legionella spp. or Legionella pneumophila

The PCR test is based on the amplification and detection of genomic sequences of a target DNA that acts as a target, in this case that of Legionella. During the test, primers (i.e., nucleic acid strands that serve as a trigger point for DNA replication) bind to a target region of the bacterium and the Taq polymerase uses these primers and deoxynucleotide triphosphates (dNTPs) to extend the DNA. creating exact copies of them (called amplicons). Subsequently, an oligonucleotide probe hybridizes to these amplicons and by emitting fluorescence, thanks to a fluorophore in its possession, it allows to “see” the presence of the bacterium’s DNA. When the target DNA is present in the sample, the fluorescence intensity increases proportionally to the increase in the amount of the amplified products and is measured by the optical module inside the thermal cycler during the hybridization step. The software combined with the instrument displays the fluorescence intensity in real time based on the number of amplification cycles. For each reaction, the software determines a Cq, i.e. the cycle from which the fluorescence significantly increases above the background noise. The value of Cq is related to the logarithm of the number of initial copies of the target sequence.

The reading of the Cq values ​​allows to detect the presence of the DNA of the Legionella bacterium and this is representative of the presence of the DNA in the original water sample. The quantification is possible thanks to the interpolation of the results obtained with a curve created specifically using standards containing various quantities of bacteria. The kits for Legionella spp and Legionella pneumophila are certified NF Validation according to the validation protocol based on the NF T90-471 standard (June 2015). The scope of the validation has been extended to the ISO / TS 12869 method (November 2012): Water quality – Detection and quantification of Legionella spp. and / or Legionella pneumophila by concentration and genic amplification by quantitative polymerase chain reaction (qPCR).

Risk Analysis
Legionella Risk Assessment

Legionellosis risk assessment is a legal obligation envisaged by Legislative Decree 81/08 and by national and regional guidelines. The international multi-year experience with the collaboration of leading companies in the sector allows us to offer you the most advanced expertise, relieving you from the burden of bureaucratic management. We perform the following:

TECHNICAL INSPECTION
  • A technical inspection with inspection and mapping of the water system, documentary collection and detailed analysis of all the elements of the system in accommodation facilities, hospitals, retirement homes, swimming pools, offices and much more.
RISK ANALYSIS
  • Risk assessment and estimation as required by the guidelines and using FMEA  techniques  that allow us to draw up a priority list (risk priority index) and define the activities immediately necessary to reduce the risk.
CREATION OF THE RISK ASSESSMENT DOCUMENT (DVR)
  • The drafting of a risk assessment manual compliant with the mandatory requirements accompanied by the maintenance and interventions register that guarantees the real-time updating of the status of your plant, meeting the legal requirements
Hygiene Protocol

Starting from the risk assessment document, we create a specific hygiene protocol for each plant with staff training, maintenance and monitoring plans.

Control audit

We perform control AUDITS on hydraulic and aeraulic systems, starting from the available documentation and detecting the actual status of maintenance and biological sampling. By adding video and photographic material, we collaborate with maintainers and plant owners to achieve the lowest possible biological risk.